• Product NameHistone H3 (mono methyl R2) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.ST0427
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF
• Species ReactivityHu, Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    HIST1 cluster, H3J antibody
  Histone gene cluster 1, H3 histone family, member E antibody
  Histone gene cluster 1, H3G antibody
  H3 histone family, member J antibody
  HIST1 cluster, H3E antibody
  HIST1 cluster, H3I antibody
  Histone gene cluster 1, H3C antibody
  FLJ92264 antibody
  H3 histone family, member A antibody
  H3 histone family, member B antibody
  H3 histone family, member C antibody
  H3 histone family, member D antibody
  H3 histone family, member F antibody
  H3 histone family, member H antibody
  H3 histone family, member I antibody
  H3 histone family, member K antibody
  H3 histone family, member L antibody
  H3 histone family, member T antibody
  H3 histone, family 3A antibody
  H3.1 antibody
  H3.3A antibody
  H3/a antibody
  H3/b antibody
  H3/c antibody
  H3/d antibody
  h3/f antibody
  H3/h antibody
  H3/i antibody
  H3/j antibody
  H3/k antibody
  H3/l antibody
  H3/t antibody
  H31_HUMAN antibody
  H3F1K antibody
  H3F3 antibody
  H3F3A antibody
  H3FA antibody
  H3FB antibody
  H3FC antibody
  H3FD antibody
  H3FF antibody
  H3FH antibody
  H3FI antibody
  H3FJ antibody
  H3FK antibody
  H3FL antibody
  HIST1 cluster, H3A antibody
  HIST1 cluster, H3B antibody
  HIST1 cluster, H3C antibody
  HIST1 cluster, H3D antibody
  HIST1 cluster, H3F antibody
  HIST1 cluster, H3G antibody
  HIST1 cluster, H3H antibody
  HIST1H3A antibody
  HIST1H3B antibody
  HIST1H3C antibody
  HIST1H3D antibody
  HIST1H3E antibody
  HIST1H3F antibody
  HIST1H3G antibody
  HIST1H3H antibody
  HIST1H3I antibody
  HIST1H3J antibody
  HIST3H3 antibody
  Histone 1, H3a antibody
  Histone 1, H3b antibody
  Histone 1, H3c antibody
  Histone 1, H3d antibody
  Histone 1, H3e antibody
  Histone 1, H3f antibody
  Histone 1, H3g antibody
  Histone 1, H3h antibody
  Histone 1, H3i antibody
  Histone 1, H3j antibody
  histone 3, H3 antibody
  histone cluster 1 H3 family member a antibody
  histone cluster 1 H3 family member b antibody
  histone cluster 1 H3 family member c antibody
  histone cluster 1 H3 family member d antibody
  histone cluster 1 H3 family member e antibody
  histone cluster 1 H3 family member f antibody
  histone cluster 1 H3 family member g antibody
  histone cluster 1 H3 family member h antibody
  histone cluster 1 H3 family member i antibody
  histone cluster 1 H3 family member j antibody
  Histone cluster 1, H3a antibody
  Histone cluster 1, H3b antibody
  Histone cluster 1, H3c antibody
  Histone cluster 1, H3d antibody
  Histone cluster 1, H3e antibody
  Histone cluster 1, H3f antibody
  Histone cluster 1, H3g antibody
  Histone cluster 1, H3i antibody
  Histone cluster 1, H3j antibody
  Histone gene cluster 1, H3 histone family, member A antibody
  Histone gene cluster 1, H3 histone family, member B antibody
  Histone gene cluster 1, H3 histone family, member C antibody
  Histone gene cluster 1, H3 histone family, member D antibody
  Histone gene cluster 1, H3 histone family, member F antibody
  Histone gene cluster 1, H3 histone family, member G antibody
  Histone gene cluster 1, H3 histone family, member H antibody
  Histone gene cluster 1, H3 histone family, member I antibody
  Histone gene cluster 1, H3 histone family, member J antibody
  Histone gene cluster 1, H3A antibody
  Histone gene cluster 1, H3B antibody
  Histone gene cluster 1, H3D antibody
  Histone gene cluster 1, H3E antibody
  Histone gene cluster 1, H3F antibody
  Histone gene cluster 1, H3H antibody
  Histone gene cluster 1, H3I antibody
  Histone gene cluster 1, H3J antibody
  Histone H 3 antibody
  Histone H3.1 antibody
  histone H3.1t antibody
  Histone H3.2 antibody
  Histone H3/a antibody
  Histone H3/b antibody
  Histone H3/c antibody
  Histone H3/d antibody
  Histone H3/f antibody
  Histone H3/h antibody
  Histone H3/i antibody
  Histone H3/j antibody
  Histone H3/k antibody
  Histone H3/l antibody
  Histone H3/m antibody
  Histone H3/o antibody
  
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• Calculated MW 15 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  
  ICC: 1:50-1:200
  
  

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

• ICC staining Histone H3(mono methyl R2) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Histone H3(mono methyl R2) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Histone H3(mono methyl R2) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting