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DNA PKcs Antibody

48923
Review: comment rank 5 pdf
  Catalog No. Package Size Price
48923-1 50ul $260.00
48923-2 100ul $390.00

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Description
  • Product Name DNA PKcs Antibody
  • Host Species Rabbit
  • Clonality Monoclonal
  • Clone No. SC57-08
  • Purification ProA affinity purified
  • Applications WB, ICC/IF, IHC
  • Species Reactivity Hu, Ms, Rt
  • Immunogen Description recombinant protein
  • Alternative Names DNA dependent protein kinase catalytic subunit antibody
    DNA PK catalytic subunit antibody
    DNA-dependent protein kinase catalytic subunit antibody
    DNA-PK catalytic subunit antibody
    DNA-PKcs antibody
    DNAPK antibody
    DNAPK catalytic subunit antibody
    DNPK 1 antibody
    DNPK1 antibody
    Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
    HYRC 1 antibody
    HYRC antibody
    HYRC1 antibody
    IMD26 antibody
    p350 antibody
    p460 antibody
    PKRDC antibody
    PRKDC antibody
    PRKDC_HUMAN antibody
    Protein Kinase DNA Activated Catalytic Polypeptide antibody
    XRCC 7 antibody
    XRCC7 antibody
  • Accession No. Swiss-Prot#:P78527
  • Calculated MW 469 kDa
  • Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage Store at -20˚C
Application Details

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Images
  • DNA PKcs Antibody - SAB | Signalway Antibody

    Western blot analysis of DNA PKcs on different lysates using anti-DNA PKcs antibody at 1/1,000 dilution. Positive control:
    Lane 1: Hela
    Lane 2: MCF-7

  • DNA PKcs Antibody - SAB | Signalway Antibody

    ICC staining DNA PKcs in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • DNA PKcs Antibody - SAB | Signalway Antibody

    ICC staining DNA PKcs in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • DNA PKcs Antibody - SAB | Signalway Antibody

    ICC staining DNA PKcs in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Background
The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.
Regerences
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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