• Product NameCD147 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JF1-045
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    5A11 antigen antibody
  5F7 antibody
  BASI_HUMAN antibody
  Basigin (Ok blood group) antibody
  Basigin antibody
  Blood brain barrier HT7 antigen antibody
  Bsg antibody
  CD 147 antibody
  CD147 antibody
  CD147 antigen antibody
  Collagenase stimulatory factor antibody
  EMMPRIN antibody
  Extracellular matrix metalloproteinase inducer antibody
  Leukocyte activation antigen M6 antibody
  M 6 antibody
  M6 antibody
  M6 leukocyte activation antigen antibody
  Neurothelin antibody
  OK antibody
  OK blood group antibody
  OK blood group antigen antibody
  TCSF antibody
  Tumor cell derived collagenase stimulatory factor antibody
  Tumor cell-derived collagenase stimulatory factor antibody
  
• Accession No. Swiss-Prot#:P35613
• UniprotP35613
• Gene ID682;
• Calculated MW 42 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

Extracellular matrix metalloproteinase inducer (EMMPRIN), also designated basigin or CD147, is involved in the regulation of matrix remodeling at the epidermal-dermal interface. EMMPRIN stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis.

• Western blot analysis of CD147 on different lysates using anti-CD147 antibody at 1/1,000 dilution. Positive control:
  Lane 1: Hela
  Lane 2: Jurkat
  Lane 3: Mouse kidney
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.
• ICC staining CD147 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD147 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting