• Product NameSFRP4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JE41-41
• PurificationProA affinity purified
• ApplicationsWB,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionFull length recombinant protein of human SFRP4.
• Alternative Names    Frizzled protein antibody
  Frizzled protein human endometrium antibody
  FRP 4 antibody
  FRP4 antibody
  FrpHE antibody
  human endometrium antibody
  PYL antibody
  Secreted frizzled-related protein 4 antibody
  sFRP-4 antibody
  Sfrp4 antibody
  SFRP4_HUMAN antibody
  
• Accession No. Swiss-Prot#:Q6FHJ7
• UniprotQ6FHJ7
• Gene ID6424;
• Calculated MW 40 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  
  
  
  FC: 1:50-1:100

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.

• Western blot analysis of SFRP4 on different lysates using anti-SFRP4 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Mouse testis
  Lane 2: A549
  Lane 3: HepG2
  Lane 4: SH-SY-5Y
  
• Flow cytometric analysis of A549 cells with SFRP4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting