Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PPP3CB in samples. An antibody specific for PPP3CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP3CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP3CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP3CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Calcineurin, a calmodulin-regulated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regulatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B (PPP3R1) in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha (PPP3CA) and beta (PPP3CB), of calcineurin A encoded by separate genes located on different human chromosomes. A third isoform, A-gamma (PPP3CC), is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs.
