Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PPP2CA in samples. An antibody specific for PPP2CA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP2CA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP2CA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP2CA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PPP2Ca encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The mammalian enzyme can be isolated as a catalytic subunit of 36 kD complexed to 1 regulatory subunit of 65 kD and to another regulatory subunit of varying molecular mass, depending on the tissue and the separation technique used. Two isoforms of the catalytic subunit of PP2A, alpha and beta, are demonstrable in many mammalian species.
