Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PODXL2 in samples. An antibody specific for PODXL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPODXL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PODXL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PODXL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PODXL2, PLEXIN-D1, GATA-2, and TMCC1 are located within the smaller critical interval of the Pakistani HCFP family. Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes. It coats the secondary foot processes of the podocytes. It is negatively charged and thus functions to keep adjacent foot processes separated, thereby keeping the urinary filtration barrier open.Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
