Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PLXNB2 in samples. An antibody specific for PLXNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Members of the B class of plexins, such as PLXNB2 is related to human SEP (PLXNB1). RT-PCR detected highest PLXNB2 expression in testis and ovary, followed by placenta, lung, liver, and kidney. Much lower expression was detected in prostate and small intestine, and little to none was detected in other tissues examined.
Ligand-induced dimerization of plexin B was sufficient to stimulate endogenous RhoA (ARHA) potently and induce the reorganization of the cytoskeleton in mammalian cells. Overexpression of the PDZ domain of PDZ-RhoGEF prevented cell rounding and neurite retraction in differentiated rat neural precursor cells induced by activation of endogenous PLXNB1 by semaphorin-4D .
