Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PIK3CD in samples. An antibody specific for PIK3CD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3CD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3CD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3CD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha, p110-beta, and p110-gamma . p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras.
Like p110-alpha and p110-beta, p110-delta binds p85 adaptor proteins and GTP-bound Ras. These 3 class I PI3Ks were indistinguishable at the level of p85 adaptor protein selection or recruitment to activated receptor complexes. However, unlike p110-alpha, p110-delta does not phosphorylate p85, but instead has an autophosphorylation activity.
