Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PGD2 in samples. An antibody specific for PGD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PGD2 is a prostaglandin which binds to the receptor PTGDR, as well as CRTH2. It is a major prostaglandin produced by mast cells C recruits Th2 cells, eosinophils, basophils. In mammalian organs, large amounts of PGD2 are found in the brain, in mast cells and found nowhere else. It is critical to development of allergic diseases such as asthma.
Causes a contraction of the bronchial airways. The concentration of PGD2 in asthma-patients is 10 times higher than in control patients, especially after it is brought into contact with allergens. Involved in the regulation of reducing body temperature, in sleep, and acts opposite to prostaglandin E2.
