• Product NameIKK a(Phospho-Thr23) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of IKKa only when phosphorylated at threonine 23.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK a.
• Target NameIKK a
• ModificationPhospho
• Alternative Names    I kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1
• Accession No. Swiss-Prot: O15111
  NCBI Protein: NP_001269.3
• UniprotO15111
• Gene ID1147;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 85kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Yuan ZQ, et al.(2002) J Biol Chem; 277(33): 29973-82.
Ozes ON, et al. (1999) Nature; 401(6748): 82-5.

• Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23) Antibody #11129.
• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Phospho-Thr23) Antibody #11129(left) or the same antibody preincubated with blocking peptide(right).
• Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK α (Phospho-Thr23) Antibody #11129.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting