• Product NameNestin Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• IsotypeIgG1
• Purificationprotein G purifed
• ApplicationsELISA, WB, IHC, IF, FC
• Species ReactivityHu
• Specificityspecific for Human Nestin denatured and native forms
• Immunogen DescriptionRecombinant nestin Protein
• Target NameNestin
• Alternative Names    NES, Nbla00170, Nestin
• Accession No. Swiss-Prot#: P48681
• UniprotP48681
• Gene ID10763;
• Concentration 0.3mg/mL
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• WB:1:500-1:5000
  IHC:1:50-1:500
  IF:1:50-1:200

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

[1] Dahlstrand J., McKay R.D.G., Zimmerman L.B., Lendahl U.Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments.J. Cell Sci.1992.103;589-597.[2]Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,

• Western BlotPositive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: NES antibody at 3µg/ml SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 178 kDaObserved band size: 260 kDa
• Immunohistochemistry of paraffin-embedded human tonsil tissue using 42024 at dilution of 1:100
• Immunohistochemistry of paraffin-embedded human kidney tissue using 42024 at dilution of 1:100
• Immunohistochemistry of paraffin-embedded human melanoma using 42024 at dilution of 1:100
• Immunofluorescent analysis of Hela cells using 42024 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
• Immunofluorescent analysis of PC-3 cells using 42024 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
• Immunofluorescent analysis of U251 cells using 42024 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
• Overlay histogram showing U251 cells stained with 42024 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein- protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
• Overlay histogram showing Hela cells stained with 42024 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein- protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting