Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KCNJ2 in samples. An antibody specific for KCNJ2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNJ2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNJ2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNJ2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:KCNJ2 is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is characterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features. The cDNA encodes a 427-amino acid protein with 2 putative transmembrane domains and an H5 region. Expression of HHIRK1 in Xenopus oocytes demonstrated strong inward rectification that was blocked by both extracellular barium and cesium ions, and they measured a single channel conductance of 30 pS.
