Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate EGFL7 in samples. An antibody specific for EGFL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEGFL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The deduced 275-amino acid protein has a calculated molecular mass of 29.8 kD. The mouse and human EGFL7 proteins both contain an N-terminal cleavable signal peptide followed by 2 epidermal growth factor (EGF)-like domains, and they share 78% amino acid identity. Northern blot analysis detected a transcript of about 1.6 kb only in mouse heart, lung, and kidney. In situ hybridization showed mouse Egfl7 expressed at embryonic day 7.5 exclusively in the primitive blood islands where the first endothelial cells differentiate, and later in endothelial cells of a wide range of tissues. Fluorescence-labeled Egfl7 was expressed in the endoplasmic reticulum of transfected mouse fibroblasts, and it was secreted into the culture medium.
