• Product NameTranscription initiation factor IIE subunit beta Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total Transcription initiation factor IIE subunit beta polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Transcription initiation factor IIE subunit beta protein
• Target NameTranscription initiation factor IIE subunit beta
• Alternative Names    General transcription factor IIE subunit 2 GTF2E2 TF2E2
• Accession No. Swiss-Prot#: P29084
• UniprotP29084
• Gene ID2961;
• Calculated MW 33kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

[1]Structure and functional properties of human general transcription factor IIE.Peterson M.G., Inostroza J., Maxon M.E., Flores O., Admon A., Reinberg D., Tjian R.Nature 354:369-373(1991) [2]Conserved sequence motifs in the small subunit of human gene

• Western blot All lanes:Transcription initiation factor IIE subunit beta antibody at 2ug/ml Lane 1:mouse brain tissue Lane 2:Hela whole cell lysate Lane 3:MCF-7 whole cell lysate Lane 4:PC-3 whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :33kDa observed band size :33kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting