Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm

Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate C5b in samples. An antibody specific for C5b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC5b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C5b is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C5b bound in the initial step. The color development is stopped and the intensity of the color is measured.