• Product NameGTPase Hras Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total GTPase Hras polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant rat GTPase HRas protein
• Target NameGTPase Hras
• Alternative Names    H-Ras-1 Transforming protein p21 c-H-ras p21ras GTPase HRas, N-terminally processed Hras Hras1
• Accession No. Swiss-Prot#: P20171
• UniprotP20171
• Gene ID293621;
• Calculated MW 21kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

[1]Nucleotide sequence of the two rat cellular rasH genes.Ruta M., Wolford R., Dhar R., Defeo-Jones D., Ellis R.W., Scolnick E.M.Mol. Cell. Biol. 6:1706-1710(1986) [2]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian

• All lanes:GTPase Hras antibody at 2ug/ml Lane1:293T whole cell lysate Lane2::mouse brain tissue Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size:21kda Observed band size:21kda

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting