• Product NameRBX1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total RBX1 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human RBX1
• Target NameRBX1
• Alternative Names    ROC1; RNF75; BA554C12.1
• Accession No. Swiss-Prot#: P62877
  Gene ID: 9978
• UniprotP62877
• Gene ID9978;
• Calculated MW 12kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg
  Lane 1-6: Jurkat, MCF7, hela and hepg2 cell, mouse brain and human placenta tissue
  Primary antibody: 1/400 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43007 at dilution 1/20.
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #43007 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting