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PRNP Antibody

43022
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  Catalog No. Package Size Price
43022 100ul $279.00

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Description
  • Product Name PRNP Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification.
  • Applications WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous levels of total PRNP protein.
  • Immunogen Type protein
  • Immunogen Description Fusion protein of human PRNP
  • Target Name PRNP
  • Alternative Names CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
  • Accession No. Swiss-Prot#: P04156
    Gene ID: 5621
  • Calculated MW 28kd
  • Concentration 1mg/ml
  • Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100

Images
  • PRNP Antibody - SAB | Signalway Antibody

    Gel: 8%SDS-PAGE
    Lysate: 40 μg
    Lane: Human fetal brain tissue
    Primary antibody: 1/400 dilution
    Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
    Exposure time: 5 minutes

  • PRNP Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #43022 at dilution 1/20.

  • PRNP Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43022 at dilution 1/20.

Background
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru.
    Please let us know if you have published research using #43022 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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