• Product NameQIP1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 459 and 471 (O00629) of KPNA4
• Target NameQIP1
• Accession No. Swiss-Prot:O00629
  Gene ID:3840
• UniprotO00629
• Gene ID3840;
• Concentration 0.7mg/ml
• Formulation Supplied in 1XPBS, 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 58kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  Immunofluorescence: 1:100-1:200
  

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: 293T
  B: HepG2
  C: Molt-4
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1500
• Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4 antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting