• Product NameSENP5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total SENP5 protein.
• Immunogen Typeprotein
• Immunogen DescriptionFusion protein of human SENP5
• Target NameSENP5
• Alternative Names    DKFZp564O1016; FLJ42398; MGC27076; SENP5; Sentrin-specific protease 5
• Accession No. Swiss-Prot#: Q96HI0
  Gene ID: 205564
• UniprotQ96HI0
• Gene ID205564;
• Calculated MW 87kd
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg
  Lane 1-3: Hela cells, K562 cells, NIH/3T3 cells
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting