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YAP (Phospho-Ser127) Conjugated Antibody

C11952
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11952-AF350 100ul $379.00
C11952-AF405 100ul $379.00
C11952-AF488 100ul $379.00
C11952-AF594 100ul $379.00
C11952-AF647 100ul $379.00
C11952-AF680 100ul $379.00
C11952-AF750 100ul $399.00
C11952-Biotin 100ul $359.00

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Description
  • Product Name YAP (Phospho-Ser127) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of YAP only when phosphorylated at serine 127.
  • Immunogen Description Peptide sequence around phosphorylation site of serine127(A-H-S(p)-S-P) derived from Human YAP .
  • Conjugates Biotin AF350 AF405 AF488 AF594 AF647 AF680 AF750
  • Alternative Names YAP65;YAP1
  • Accession No. Swiss-Prot#:P46937
    NCBI Gene ID:10413
    NCBI mRNA#:NM_001130145.2
    NCBI Protein#:NP_001123617.1
  • Calculated MW 65
  • Concentration
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4˚Cin dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin
ELISA: 1: 200 - 1: 1,000
Western Blot: 1: 200 - 1: 1,000
IHC/ICC: 1: 100 - 1: 500
Flow Cytometry: 1: 50 - 1: 250
IF: 1: 100 - 1: 500
AF350 conjugated: most apllications: 1: 50 - 1: 250
AF405 conjugated: most apllications: 1: 50 - 1: 250
AF488 conjugated: most apllications: 1: 50 - 1: 250
AF594 conjugated: most apllications: 1: 50 - 1: 250
AF647 conjugated: most apllications: 1: 50 - 1: 250
AF680 conjugated: Western blot: 1: 100-1,000
AF750 conjugated: Western blot: 1: 100-1,000
desc_detail:Excitation/Emission
Alexa Fluor 350 (AF350): 346nm/442nm
Alexa Fluor 405 (AF405): 401nm/421nm
Alexa Fluor 488 (AF488): 493nm/519nm
Alexa Fluor 594 (AF594): 591nm/614nm
Alexa Fluor 647 (AF647): 651nm/667nm
Alexa Fluor 680 (AF680): 679nm/702nm
Alexa Fluor 750 (AF750): 749nm/775nm

Product Description

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Background

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.

    Please let us know if you have published research using #C11952 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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