• Product NameTCF7L1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TCF7L1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human TCF7L1
• Target NameTCF7L1
• Alternative Names    TCF3; TCF-3
• Accession No. Swiss-Prot#: Q9HCS4
  NCBI Gene ID: 83439
• UniprotQ9HCS4
• Gene ID83439;
• Calculated MW 63kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane: Hepg2 cell lysate,
  Primary antibody:TCF7L1 antibody at dilution 1/200 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 20 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TCF7L1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using TCF7L1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting