• Product NameWEE1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total WEE1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human WEE1
• Target NameWEE1
• Alternative Names    WEE1A; WEE1hu
• Accession No. Swiss-Prot#: P30291
  NCBI Gene ID: 7465
• UniprotP30291
• Gene ID7465;
• Calculated MW 72kd
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane: 293T cell lysate,
  Primary antibody:WEE1 antibody at dilution 1/200,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 1 second
• The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using WEE1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using WEE1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting