• Product NameWIPI2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total WIPI2 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human WIPI2
• Target NameWIPI2
• Alternative Names    Atg21; ATG18B; CGI-50; WIPI-2
• Accession No. Swiss-Prot#: Q9Y4P8
  NCBI Gene ID: 26100
• UniprotQ9Y4P8
• Gene ID26100;
• Calculated MW 49kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane 1-2: Human lung tissue and Human placenta tissue lysates,
  Primary antibody:WIPI2 antibody at dilution 1/300,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 2 minutes
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using WIPI2 Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using WIPI2 Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting