• Product NameNF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total NF1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human NF1
• Target NameNF1
• Alternative Names    WSS; NFNS; VRNF
• Accession No. Swiss-Prot#: P21359
  NCBI Gene ID: 4763
• UniprotP21359
• Gene ID4763;
• Calculated MW 319kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane: NIH/3T3 cell lysate,
  Primary antibody:NF1 antibody at dilution 1/250,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 1 second
• The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using NF1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting