• Product NameDSCC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DSCC1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human DSCC1
• Target NameDSCC1
• Alternative Names    DCC1
• Accession No. Swiss-Prot#: Q9BVC3
  NCBI Gene ID: 79075
• UniprotQ9BVC3
• Gene ID79075;
• Calculated MW 45kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 10-50

Loads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane: 231 cell lysate,
  Primary antibody:DSCC1 antibody at dilution 1/200,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 90 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using DSCC1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using DSCC1 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting