• Product NameeNOS(Phospho-Ser1177) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of eNOS only when phosphorylated at serine 1177.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS.
• Target NameeNOS
• ModificationPhospho
• Alternative Names    Constitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII
• Accession No. Swiss-Prot: P29474
  NCBI Protein: NP_000594.2
• UniprotP29474
• Gene ID4846;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 133kd

  Western blotting: 1:500~1:1000

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

• Western blot analysis of extracts from 3T3 cells untreated and treated with EGF, HUVEC cells treated with VEGF, JK cells untreated and treated with serum using eNOS (Phospho-Ser1177) Antibody #11156.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting