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Beta Actin antibody

22957
Review: comment rank 5 pdf
  Catalog No. Package Size Price
22957 100ul $390.00

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Description
  • Product Name Beta Actin antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Purified by antigen-affinity chromatography.
  • Applications WB IHC IF
  • Species Reactivity Hu
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide contain a sequence corresponding to a region within amino acids 309 and 368 of Human ACTB
  • Target Name Beta Actin
  • Accession No. NCBI Gene ID: 60
    NCBI mRNA#: NM_001101
    NCBI Protein#: NP_001092
  • Concentration 1mg/ml
  • Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
  • Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details

Predicted MW: 42kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
 

Images
  • Beta Actin antibody - SAB | Signalway Antibody

    Sample (30 ug of whole cell lysate)
    A: A431
    B: H1299
    10% SDS PAGE
    Primary antibody diluted at 1: 1000

  • Beta Actin antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded Prostate, using beta Actin antibody(10 ug/ml).

  • Beta Actin antibody - SAB | Signalway Antibody

    Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

    Please let us know if you have published research using #22957 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
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