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GAPDH antibody

23001
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  Catalog No. Package Size Price
23001 100ul $390.00

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Description
  • Product Name GAPDH antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Purified by antigen-affinity chromatography.
  • Applications WB IHC IF
  • Species Reactivity Hu
  • Immunogen Type Recombinant protein
  • Immunogen Description Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 323 of GAPDH
  • Target Name GAPDH
  • Alternative Names G3PD; GAPD; MGC88685
  • Accession No. NCBI Gene ID: 2597
    NCBI mRNA#: NM_002046
    NCBI Protein#: NP_002037
  • Concentration 1mg/ml
  • Formulation Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
  • Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
Application Details

Predicted MW: 36kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200

Images
  • GAPDH antibody - SAB | Signalway Antibody

    Sample (30 ug of whole cell lysate)
    A: Jurkat
    B: Raji
    C: 293T
    D: A431
    E: HeLa
    F: HepG2
    G: H1299
    H: HCT116
    I: MCF-7
    J: NT2D1
    K: PC-3
    L: U87-MG
    10% SDS PAGE
    Primary antibody diluted at 1: 10000

  • GAPDH antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of Salivary gland cancer, using GAPDH antibody at 1: 500 dilution.

  • GAPDH antibody - SAB | Signalway Antibody

    Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1: 500 dilution and alpha-tubulin antibody (red) at 1: 2000.

Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

    Please let us know if you have published research using #23001 so that we can cite your reference.
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      Application:
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    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
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    • NCNegative Control
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