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NFκB-p65(Phospho-Thr435) Conjugated Antibody

C11012
Review: comment rank 5 pdf
  Catalog No. Package Size Price
C11012-AF350 100ul $379.00
C11012-AF405 100ul $379.00
C11012-AF488 100ul $379.00
C11012-AF594 100ul $379.00
C11012-AF647 100ul $379.00
C11012-AF680 100ul $379.00
C11012-AF750 100ul $399.00
C11012-Biotin 100ul $359.00

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Description
  • Product Name NFκB-p65(Phospho-Thr435) Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of NF-κB p65 only when phosphorylated at threonine 435.
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 435(E-G-T(p)-L-S) derived from Human NFκB-p65.
  • Conjugates Biotin AF350 AF405 AF488 AF594 AF647 AF680 AF750
  • Alternative Names NFKB3;RELA;TF65;Transcription factor p65;p65
  • Accession No. Swiss-Prot#:Q04206
    NCBI Gene ID:5970
    NCBI mRNA#:NM_001145138.1
    NCBI Protein#:NP_001138610.1
  • Calculated MW 65
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 10mg/ml Bovine Serum Albumin, 0.05% Sodium Azide
  • Storage Store at 4˚C in dark for 6 months
Application Details

Biotin conjugated: Working with enzyme-conjugated streptavidin
ELISA: 1: 200 - 1: 1,000
Western Blot: 1: 200 - 1: 1,000
IHC/ICC: 1: 100 - 1: 500
Flow Cytometry: 1: 50 - 1: 250
IF: 1: 100 - 1: 500
AF350 conjugated: most apllications: 1: 50 - 1: 250
AF405 conjugated: most apllications: 1: 50 - 1: 250
AF488 conjugated: most apllications: 1: 50 - 1: 250
AF594 conjugated: most apllications: 1: 50 - 1: 250
AF647 conjugated: most apllications: 1: 50 - 1: 250
AF680 conjugated: Western blot: 1: 100-1,000
AF750 conjugated: Western blot: 1: 100-1,000
desc_detail:Excitation/Emission
Alexa Fluor 350 (AF350): 346nm/442nm
Alexa Fluor 405 (AF405): 401nm/421nm
Alexa Fluor 488 (AF488): 493nm/519nm
Alexa Fluor 594 (AF594): 591nm/614nm
Alexa Fluor 647 (AF647): 651nm/667nm
Alexa Fluor 680 (AF680): 679nm/702nm
Alexa Fluor 750 (AF750): 749nm/775nm

Product Description
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Background
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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