• Product NameEIF4G3 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionRecombinant fusion protein of human EIF4G3 (NP_003751.2).
• Alternative Names    EIF4G3; eIF-4G 3; eIF4G 3; eIF4GII; eIF-4G3; eIF4G3; eukaryotic translation initiation factor 4 gamma 3
• Accession No. Swiss-Prot#:O43432
  NCBI Gene ID:8672
• UniprotO43432
• Gene ID8672;
• Calculated MW 250kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p.

• Western blot analysis of extracts of various cell lines, using EIF4G3 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting