• Product NameEYA3 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Rat
• Immunogen DescriptionRecombinant fusion protein of human EYA3 (NP_001981.2).
• Alternative Names    EYA3; eyes absent homolog 3
• Accession No. Swiss-Prot#:Q99504
  NCBI Gene ID:2140
• UniprotQ99504
• Gene ID2140;
• Calculated MW Refer to figures
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.

• Immunofluorescence analysis of U2OS cells using EYA3 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using EYA3 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting