• Product NameMAPK6 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human MAPK6 (NP_002739.1).
• Alternative Names    MAPK6; ERK3; HsT17250; PRKM6; p97MAPK; mitogen-activated protein kinase 6
• Accession No. Swiss-Prot#:Q16659
  NCBI Gene ID:5597
• UniprotQ16659
• Gene ID5597;
• Calculated MW 120kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:200 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

• Western blot analysis of extracts of various cell lines, using MAPK6 at 1:1000 dilution.
• Immunofluorescence analysis of L929 cells using MAPK6 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blot analysis of extracts from normal (control) and MAPK6 knockout (KO) HeLa cells, using MAPK6 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting