• Product NameP90RSK Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic synthetic peptide of human P90RSK (NP_002944.2).
• Alternative Names    RPS6KA1; HU-1; MAPKAPK1A; RSK; RSK1; p90Rsk; ribosomal protein S6 kinase A1
• Accession No. Swiss-Prot#:Q15418
  NCBI Gene ID:6195
• UniprotQ15418
• Gene ID6195;
• Calculated MW 90kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:100 - 1:200
  IF 1:50 - 1:200

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

• Immunohistochemistry of paraffin-embedded mouse spleen using RPS6KA1 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using P90RSK at 1:1000 dilution.
• Immunofluorescence analysis of L929 cells using RPS6KA1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using RPS6KA1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting