• Product NameDiMethyl-DNMT3A-K44 pAb
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsIF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).
• Alternative Names    Dnmt3a; MmuIIIA; DNMT3A2; M.HsaIIIA; TBRS; DNA methyltransferase 3A
• Accession No. Swiss-Prot#:O88508
  NCBI Gene ID:13435
• UniprotO88508
• Gene ID13435;
• Calculated MW
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• IF 1:50 - 1:200

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

• Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting