• Product NameVAT1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human VAT1.
• Alternative Names    VAT1; VATI; vesicle amine transport 1
• Accession No. Swiss-Prot#:Q99536
  NCBI Gene ID:10493
• UniprotQ99536
• Gene ID10493;
• Calculated MW Refer to figures
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins.

• Western blot analysis of extracts of various cell lines, using VAT1 at 1:1000 dilution.
• Western blot analysis of extracts from normal (control) and VAT1 knockout (KO) 293T cells, using VAT1 at 1:1000 dilution.
• Immunofluorescence analysis of L929 cells using VAT1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting