• Product NameCSPG4 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human NG2/CSPG4 (NP_001888.2).
• Alternative Names    CSPG4;HMW-MAA;MCSP;MCSPG;MEL-CSPG;MSK16;NG2
• Accession No. Uniprot:Q6UVK1
  GeneID:1464
• UniprotQ6UVK1
• Gene ID1464
• Calculated MW 320kDa
• SDS-PAGE MW 250KDa-450KDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

• Western blot analysis of extracts of A375 cells, using NG2/CSPG4 antibody.
• Immunofluorescence analysis of A-375 cells using [KO Validated] NG2/CSPG4 Rabbit pAb.
• Western blot analysis of extracts of various cell lines, using NG2/CSPG4 antibody.
• Immunohistochemistry of paraffin-embedded Mouse brain using NG2/CSPG4 antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting