• Product NameVARS Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human VARS (NP_006286.1).
• Alternative Names    VARS; G7A; VARS1; VARS2; valine--tRNA ligase
• Accession No. Swiss-Prot#:P26640
  NCBI Gene ID:7407
• UniprotP26640
• Gene ID7407;
• Calculated MW 140kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.

• Immunofluorescence analysis of L929 cells using VARS Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human breast cancer using VARS at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using VARS at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using VARS at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat brain using VARS at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting