• Product NameARHGEF1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Rat
• Immunogen DescriptionRecombinant fusion protein of human ARHGEF1 (NP_004697.2).
• Alternative Names    ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5; rho guanine nucleotide exchange factor 1
• Accession No. Swiss-Prot#:Q92888
  NCBI Gene ID:9138
• UniprotQ92888
• Gene ID9138;
• Calculated MW 102kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:200

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

• Western blot analysis of extracts of various cell lines, using ARHGEF1 at 1:3000 dilution.
• Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 at 1:500 dilution.
• Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting