• Product NameEIF4E2 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human EIF4E2 (NP_004837.1).
• Alternative Names    EIF4E2;4E-LP;4EHP;EIF4EL3;IF4e;h4EHP
• Accession No. Uniprot:O60573
  GeneID:9470
• UniprotO60573
• Gene ID9470
• Calculated MW 28kDa
• SDS-PAGE MW 27KDa/28KDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:200 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3, suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity. In P-bodies, component of a complex that promotes miRNA-mediated translational repression.

• Western blot analysis of extracts of various cell lines, using EIF4E2 antibody.
• Western blot analysis of extracts of various cell lines, using EIF4E2 antibody.
• Immunofluorescence analysis of U-2 OS cells using EIF4E2 Rabbit pAb.
• Immunofluorescence analysis of NIH-3T3 cells using EIF4E2 Rabbit pAb.
• Immunofluorescence analysis of C6 cells using EIF4E2 Rabbit pAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting