• Product NameNCL Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse
• Immunogen DescriptionRecombinant fusion protein of human NCL (NP_005372.2).
• Alternative Names    NCL;C23;Nsr1;nucleolin
• Accession No. Uniprot:P19338
  GeneID:4691
• UniprotP19338
• Gene ID4691
• Calculated MW 110kDa
• SDS-PAGE MW 110kDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

• Immunohistochemistry of paraffin-embedded human liver cancer using NCL Rabbit pAb.
• Western blot analysis of extracts of various cell lines, using NCL antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting