• Product NameNELFE Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman
• Immunogen DescriptionRecombinant fusion protein of human NELFE (NP_002895.3).
• Alternative Names    NELFE; D6S45; NELF-E; RD; RDBP; RDP; negative elongation factor E
• Accession No. Swiss-Prot#:P18615
  NCBI Gene ID:7936
• UniprotP18615
• Gene ID7936;
• Calculated MW 43kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

• Western blot analysis of extracts of various cell lines, using NELFE at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using NELFE .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting