• Product NameLC3B Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human LC3B
• Alternative Names    MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta
• Accession No. Swiss-Prot#:Q9GZQ8
  NCBI Gene ID:81631
• UniprotQ9GZQ8
• Gene ID81631;
• Calculated MW 14kDa, 16kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

• Western blot analysis of extracts of various cell lines, using LC3B at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) for 20 hours. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.
• Western blot analysis of extracts of pig liver, using LC3B .
• Immunohistochemistry of paraffin-embedded rat heart using LC3B at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human lung cancer using LC3B at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using LC3B at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using LC3B at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat brain using LC3B at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human breast cancer using LC3B at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using LC3B at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of rat brain using LC3B at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting