• Product NameBCS1L Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human BCS1L (NP_004319.1).
• Alternative Names    BCS1L; BCS; BCS1; BJS; FLNMS; GRACILE; Hs.6719; MC3DN1; PTD; h-BCS; h-BCS1; mitochondrial chaperone BCS1
• Accession No. Swiss-Prot#:Q9Y276
  NCBI Gene ID:617
• UniprotQ9Y276
• Gene ID617;
• Calculated MW 48kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Several alternatively spliced transcripts encoding two different isoforms have been described.

• Western blot analysis of extracts of various cell lines, using BCS1L at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using BCS1L at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human stomach using BCS1L at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat brain using BCS1L at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting