• Product NameCHD1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic synthetic peptide of human CHD1 (NP_001261.2).
• Alternative Names    CHD1; chromodomain-helicase-DNA-binding protein 1
• Accession No. Swiss-Prot#:O14646
  NCBI Gene ID:1105
• UniprotO14646
• Gene ID1105;
• Calculated MW 197kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:100 - 1:200

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

• Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 at 1:500 dilution.
• Western blot analysis of extracts of various cell lines, using CHD1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded mouse brain using CHD1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver damage using CHD1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat brain using CHD1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting