• Product NamePOR Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human POR (NP_000932.3).
• Alternative Names    POR; CPR; CYPOR; P450R; cytochrome p450 oxidoreductase
• Accession No. Swiss-Prot#:P16435
  NCBI Gene ID:5447
• UniprotP16435
• Gene ID5447;
• Calculated MW 77kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:200

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

• Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR at 1:1000 dilution.
• Immunofluorescence analysis of C6 cells using POR Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of HeLa cells using POR Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH-3T3 cells using POR Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using POR at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human esophagus using POR at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting