• Product NameMETTL3 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human METTL3 (NP_062826.2).
• Alternative Names    METTL3; IME4; M6A; MT-A70; Spo8; hMETTL3; methyltransferase like 3
• Accession No. Swiss-Prot#:Q86U44
  NCBI Gene ID:56339
• UniprotQ86U44
• Gene ID56339;
• Calculated MW 80kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200
  IP 1:20 - 1:50

This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

• Western blot analysis of extracts of various cell lines, using METTL3 at 1:1000 dilution.
• Immunofluorescence analysis of C6 cells using METTL3 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of HeLa cells using METTL3 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH/3T3 cells using METTL3 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Confocal immunofluorescence analysis of Hela cells using METTL3 Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.
• Confocal immunofluorescence analysis of U-2 OS cells using METTL3 Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting