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MEP1B Conjugated Antibody

C27286
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  Catalog No. Package Size Price
C27286-AF350 100ul $399.00
C27286-AF405 100ul $399.00
C27286-AF488 100ul $399.00
C27286-AF555 100ul $399.00
C27286-AF594 100ul $399.00
C27286-AF647 100ul $399.00
C27286-AF680 100ul $399.00
C27286-AF750 100ul $399.00
C27286-Biotin 100ul $399.00

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Description
  • Product Name MEP1B Conjugated Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Isotype IgG
  • Purification Affinity purification
  • Applications most applications
  • Species Reactivity Ms
  • Immunogen Description Recombinant fusion protein of human MEP1B (NP_005916.2).
  • Conjugates Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
  • Excitation Emission AF350: 346nm/442nm
    AF405: 401nm/421nm
    AF488: 493nm/519nm
    AF555: 555nm/565nm
    AF594: 591nm/614nm
    AF647: 651nm/667nm
    AF680: 679nm/702nm
    AF750: 749nm/775nm
  • Alternative Names MEP1B; meprin A subunit beta
  • Accession No. Swiss-Prot#:Q16820
    NCBI Gene ID:4225
  • Calculated MW 105kDa
  • Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
  • Storage Store at 4˚C in dark for 6 months
Application Details

Suggested Dilution:
AF350 conjugated: most applications: 1: 50 - 1: 250
AF405 conjugated: most applications: 1: 50 - 1: 250
AF488 conjugated: most applications: 1: 50 - 1: 250
AF555 conjugated: most applications: 1: 50 - 1: 250
AF594 conjugated: most applications: 1: 50 - 1: 250
AF647 conjugated: most applications: 1: 50 - 1: 250
AF680 conjugated: most applications: 1: 50 - 1: 250
AF750 conjugated: most applications: 1: 50 - 1: 250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

Background
Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
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