• Product NameCHFR Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CHFR protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to internal residues of human CHFR
• Target NameCHFR
• Alternative Names    RNF116; RNF196
• Accession No. Swiss-Prot:Q96EP1
  NCBI Gene ID:55743
  NCBI Protein:NP_001154816
• UniprotQ96EP1
• Gene ID55743;
• Calculated MW 73 kDa
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1: 20-100

This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.

• Gel: 8%SDS-PAGE
  , Lysate: 40 ¦Ìg,, Lane 1-6: Hela,Jurkat,A549,HepG2,293T and 231 cell lysates,
  Primary antibody: 46489(CHFR Antibody) at dilution 1/300,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 10 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 46489(CHFR Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting